FastQCFastQC Report
Mon 6 Jun 2016
ERR1378594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611404
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA27190.44471413337171495No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA20370.33316759458557677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAC2350.042.12811718
AGGCGGT2700.041.6671234
GGCGGTC2750.040.9095435
CGTCATA2400.040.3129424
GACGTCA2400.040.30964322
ACGTCAT2400.040.30964323
CGGTATT2800.040.1790124
CCGATGA2450.039.49022717
GATGACG2450.039.49022719
TGACGTC2450.039.48721
ATGACGT2450.039.48720
AATTCGG2850.039.47088620
TTCGGTA2850.039.47088622
TCGGTAT2850.039.47088623
CGGGGAA2950.038.89872715
TAAGCCG2500.038.7004213
GCGGTCT2950.038.13600536
AGCCGAT2550.037.94159315
TCGGGGA3050.037.62336314
AAGCCGA2600.037.21194514
Produced by FastQC (version 0.11.5)