FastQCFastQC Report
Mon 6 Jun 2016
ERR1378592.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378592.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505714
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA23780.4702262543651155No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA16070.3177685411121701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG2000.043.87307413
CCGATGA2100.042.8552617
AGCCGAT2150.040.8121615
ACGTCAT2150.040.8121623
CGTCATA2150.040.8121624
GATGACG2250.039.9982419
ATGACGT2300.039.12871620
GACGTCA2250.038.99828322
CGATGAC2250.038.99828318
TGACGTC2300.038.15049721
GCCGATG2300.038.15049716
AAGCCGA2300.038.15049714
TCGGTAT1950.036.92145523
CGGTATT2000.035.99841724
CTAGGCG250.002106449635.99841730
GCGGTCT2050.035.12040736
CGGGGAA2050.035.12040715
AATTCGG2050.035.12040720
CTCGTAT458.690113E-734.99846339
TATGCCG458.690113E-734.99846343
Produced by FastQC (version 0.11.5)