FastQCFastQC Report
Mon 6 Jun 2016
ERR1378590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690021
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA29780.4315810678225735No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24010.3479604243928808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3000.040.49882513
TTCGGTA3300.040.2261122
AATTCGG3450.039.129320
GACGTCA3150.038.57031222
GCCGATG3150.038.57031216
TCGGTAT3450.038.47714623
CGGGGAA3550.038.02706515
AGCCGAT3200.037.96764815
AAGCCGA3200.037.96764814
CGTCATA3200.037.96764824
ATTCGGT3500.037.9274721
CGGTATT3500.037.9274724
CCGATGA3300.037.49891317
TCGGGGA3600.037.49891314
CGGTCTC3650.036.9852337
GCGGTCT3700.036.4854336
GAATTCG3700.036.4854319
ACGTCAT3350.036.26760523
TCCTCGG3450.035.86852611
CGATGAC3450.035.2163718
Produced by FastQC (version 0.11.5)