FastQCFastQC Report
Mon 6 Jun 2016
ERR1378589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949392
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA30760.32399683165647064No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24790.2611144816893338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3300.040.90756213
CGGTATT3050.040.5722524
TCGGTAT3150.039.28424523
TTCGGTA3200.038.67042522
GCCGATG3550.038.66052216
GATGACG3500.038.56998419
CGTCATA3500.038.56998424
ACGTCAT3650.038.21774723
GCGGTCT3250.038.07549736
GGCGGTC3250.038.07549735
AATTCGG3250.038.07549720
AGCCGAT3550.038.02674515
AAGCCGA3550.038.02674514
TCGGGGA3300.037.49859614
ATGACGT3600.037.49859220
CGATGAC3700.037.09320418
AGGCGGT3350.036.93891534
CCGATGA3800.036.1170717
CGGGGAA3500.035.9986515
CGGTCTC3450.035.86821737
Produced by FastQC (version 0.11.5)