FastQCFastQC Report
Mon 6 Jun 2016
ERR1378586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138252
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA36940.3245327045329154No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29980.26338631515692484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG3850.041.49006313
CGATGAC3950.040.43968218
CCGATGA4000.039.9341917
CGTCATA4050.039.44117424
TTCGGTA4000.039.37173522
TCGGTAT4000.039.37173523
TGACGTC4100.038.9601821
ATGACGT4100.038.9601820
TCGGGGA4150.038.4907814
GCCGATG4150.038.4907816
GATGACG4250.038.11448719
AGCCGAT4200.038.0325615
ACGTCAT4200.038.0325623
GGCGGTC4300.037.6712935
GACGTCA4250.037.58511722
AGGCGGT4400.037.32644734
GAATTCG4300.037.14808319
GCGGTCT4350.036.7210936
CGGTATT4300.036.6248724
TTCCTCG3900.036.34313610
Produced by FastQC (version 0.11.5)