FastQCFastQC Report
Fri 10 Feb 2017
ERR1148526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3964465
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478071.2058878057947289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260440.6569360556846888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234200.5907480580607977No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA168570.4252023917476885No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT138840.35021118864714407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.24590959940370263No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76390.19268678119241814No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62820.15845769857975792No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59000.148822098315914No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50330.12695281708881276No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49170.12402682329141512No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA48350.12195844836566852No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45570.11494615288569832No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC45320.11431555077419023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.11411375809850761No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44150.11136433289233225No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42800.10795908149018846No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41780.10538622487523538No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40840.10301516093596488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4000.047.5699422
GGGTTAG15750.037.39951
ATGGGGG94900.033.834465
CATGGGG157250.033.6913764
CATGGGT105700.033.4713864
GTTAGAG19550.031.935543
GGTTAGA19200.031.5894172
ATGGGTG57850.031.5548935
ATCACGC6250.031.395373
TCACGCA6700.030.1742044
GTATCAA672400.029.2009771
TTAGAGC19800.028.8295444
TGGGGGG83850.028.4348626
TAGAGCA22800.028.1657055
AACGCAG712350.027.7612256
CGCATAG4350.027.3559551
TATAACG3200.026.0148142
TATCAAC771000.025.4739842
ATCAACG770350.025.4022083
ACGCAGA782850.025.2231967
Produced by FastQC (version 0.11.5)