FastQCFastQC Report
Fri 10 Feb 2017
ERR1148523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811347
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391161.0263038238187183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216480.5679881679626652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200980.5273201311767205No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA124610.32694477831590774No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT103310.27105902453909336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80400.21094904242515833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55810.14643116987248866No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA55600.14588018356764682No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49510.1299015807272337No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44410.11652048475250351No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.10691758058240303No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.10620916961903495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG95950.034.7657975
CATGGGG148300.033.92064
TATAACG3750.033.2983252
TGGGGGG83200.029.9434786
GGGTTAG14800.029.7525061
GTATCAA542650.029.7387961
AACGCAG564000.028.6010656
GTGGTTA23250.026.873231
ATATGTA22350.026.6241651
TATCAAC619350.025.9312112
ACGCAGA623750.025.842267
GGTTAGA16600.025.790442
ATCAACG623900.025.6849143
CGCAGAG629050.025.6528858
TCAACGC629500.025.6453384
CAACGCA633850.025.478725
TATGTAA23250.025.3192
GTTAGAG17850.025.3168533
AGTACTT264300.024.71334512-13
GCAGAGT653050.024.7101279
Produced by FastQC (version 0.11.5)