FastQCFastQC Report
Fri 10 Feb 2017
ERR1148522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854041
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387441.0052825073734295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220690.5726197515802245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194690.5051580925060216No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA178510.4631761831283061No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT142890.37075371019664816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81280.2108955249827389No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG69710.1808750866947186No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62420.1619598753619902No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT54340.14099486746508405No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC54020.14016457012263234No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA52830.13707690188038996No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50370.13069399106029228No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47220.1225207515955331No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC45970.11927740260158104No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC45760.11873251997059708No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC39420.10228225387327224No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39400.10223036028936901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4950.038.444152
ATGGGGG85250.034.2505045
CATGGGG141050.033.0539134
GGGTTAG11350.029.3602331
TGGGGGG72000.027.831946
CATGGGT37850.026.080874
CGCATAG5500.024.8847031
AACGCAG629300.024.6242336
GTATCAA632000.024.2641261
TCACGCA8600.024.20194
ACATGGG567500.023.5040823
GTAAGTA29550.023.143332
AGTACTT271100.023.10799812-13
GAGTACT261250.022.6590812-13
GTACATG631850.022.565251
TACATGG632150.022.4928722
ACGCAGA688350.022.4513787
CGCAGAG693600.022.2985868
GGTTAGA13450.022.1071722
TATGTAA19100.022.1060162
Produced by FastQC (version 0.11.5)