FastQCFastQC Report
Fri 10 Feb 2017
ERR1148521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295208
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401501.7492967957588157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257521.1219898153021426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188790.8225398308127194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.3648035385028285No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA48860.21287830993966558No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA44390.19340295084367082No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.17902516896072165No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA39480.1720105541632828No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38360.16713082213028188No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA31710.13815741318433883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.13218845525111447No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA29430.12822367297430123No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA29370.12796225875824763No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.12465101202156842No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26040.11345376976727164No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23120.10073161125266207No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.10007807571252801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA403300.037.791
AACGCAG404650.037.778876
ATGGGGG61400.034.7680935
GGTATCA264100.033.890271
TATCAAC461250.033.0563852
ACGCAGA462050.033.0084277
CGCAGAG463250.032.935768
ATCAACG464650.032.7618833
TCAACGC470450.032.459094
CATGGGG107950.032.444984
GCAGAGT477250.031.9695959
CAACGCA478450.031.9287855
ATATGTA10650.031.2920481
GGGTTAG5000.029.7553861
TGGGGGG53700.029.0103846
TATGTAA11900.028.4840452
TATAAGT4950.027.629784
CATGGGT31850.026.5114674
GTACATG370850.026.204961
GCGTATC2057.762901E-826.126681
Produced by FastQC (version 0.11.5)