FastQCFastQC Report
Fri 10 Feb 2017
ERR1148520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189549
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334070.7973889313623018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186830.4459429881354771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164220.39197536536748945No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120140.2867611764416647No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT102690.24510991517225364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68580.16369303712643055No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG58200.13891710062348No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47900.11433211546159265No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44570.10638376588983683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3950.039.1398622
CATGGGG142450.034.4377334
ATGGGGG92350.033.6106265
TGGGGGG79450.029.636276
GTATCAA487800.027.6804261
AACGCAG521400.026.160426
CATGGGT55550.025.369234
ACAACGC7300.025.251193
GGGTTAG14450.025.1212961
ACATGGG494250.024.747463
TCAACGC558450.024.3088634
ATCAACG554750.024.2351823
CAACGCA563750.024.1225175
TATCAAC563350.023.9918752
TACAACG6950.023.9561062
GTACATG535250.023.8034571
ACGCAGA575000.023.701137
AGTACTT227800.023.67479712-13
TACATGG539400.023.6240442
CGCAGAG580600.023.5442188
Produced by FastQC (version 0.11.5)