FastQCFastQC Report
Fri 10 Feb 2017
ERR1148517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6375501
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480680.7539485916479348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250740.3932867393480136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234200.36734368012804014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140010.21960627094247182No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT117610.18447177719837232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97740.15330559904233407No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC81870.1284134376263136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG155800.035.1874285
CATGGGG233550.034.8281444
GTATCAA621000.031.9143871
GGGTTAG22800.031.0538581
TGGGGGG129100.030.6724346
AACGCAG669550.029.7660526
ATCAACG717100.027.4953253
TCAACGC723600.027.4866394
TATCAAC723500.027.3842432
CAACGCA729600.027.2442995
TATCACG5950.026.9824352
ACGCAGA743500.026.7174957
CGCAGAG753500.026.402378
ACATGGG656900.026.1047823
GTACATG710800.025.6056351
TACAACG7700.025.4834082
TACATGG715300.025.3456542
CATGGGT75200.025.2230114
GCAGAGT791750.025.1418729
GGTTAGA26200.024.9646632
Produced by FastQC (version 0.11.5)