FastQCFastQC Report
Fri 10 Feb 2017
ERR1148516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537425
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368950.8131263877639852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201800.4447456431786751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183090.4035108018314353No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA164230.36194537650760067No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT147090.32417064744871815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72040.15876846449252605No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70950.15636622092927155No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60260.1328066028639592No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58820.1296329966886505No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53560.11804051857606462No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50490.1112745665217607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47460.10459677019454866No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46860.10327443428817006No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA45710.1007399571342777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG117600.036.052785
CATGGGG171900.035.3188364
TATCACG4400.031.0846562
TGGGGGG103300.031.082886
GGGTTAG14900.029.1563451
CATGGGT62200.028.2025364
GTATCAA577950.026.9160821
GTGGTTA26600.025.9521221
AACGCAG612050.025.686356
GGTTAGA17600.025.6786292
ACATGGG588650.025.1131113
GTAAGTA42700.025.0677592
TATAACG4600.024.5621682
TACATGG652650.024.0452862
GTACATG652950.024.0340351
TATCAAC655150.023.7810732
ATCAACG656850.023.6101063
ACGCAGA667350.023.5667577
ATATGTA28100.023.5078641
TCAACGC663750.023.4721744
Produced by FastQC (version 0.11.5)