FastQCFastQC Report
Fri 10 Feb 2017
ERR1148515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386122
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471610.6385082726767849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242150.32784457121071114No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA238200.3224967039537121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234600.3176226983523966No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT203090.2749616104364374No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100650.13626907327011387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96320.13040672764408712No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC86890.11763954074952998No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85280.1154597771333861No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80690.10924541999170878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG209000.029.6738534
ATGGGGG125900.029.2348485
TATCACG7600.028.9489542
GGGTTAG28200.027.6424031
GTATCAA790600.024.7699221
TGGGGGG101800.024.1219146
AACGCAG827800.023.9181886
ACATGGG768150.022.7039763
AGTACTT337950.022.6353612-13
GGTTAGA31850.022.4035362
CATGGGT79350.022.330854
ATCAACG882750.021.9793743
TCAACGC889650.021.9425774
ACGCAGA902850.021.890467
TATCAAC894550.021.8494172
CAACGCA896350.021.7984645
GTACATG870700.021.7872981
TACATGG871750.021.7524072
GAGTACT323800.021.6320912-13
CGCAGAG916300.021.6210488
Produced by FastQC (version 0.11.5)