FastQCFastQC Report
Fri 10 Feb 2017
ERR1148513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714945
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357340.9618984937865835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194670.5240185251733201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177250.47712684844593933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA157110.42291339441095355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT131090.35287198060805747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73740.19849553627308078No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73500.1978494970988803No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59110.15911406494577982No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58520.15752588530920378No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53150.14307075878646924No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG48420.13033840339493585No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48100.1294770178293353No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA46280.12457788742498206No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43750.11776755779695257No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39570.10651570884629517No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG39220.10557356838391954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.10463142792154392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG105000.037.5463565
CATGGGG149700.036.1859664
TATCACG2850.033.382942
TGGGGGG90000.032.8338976
GGGTTAG18150.031.8028581
GTTAGAG19950.029.5077383
GTGGTTA24800.028.5539631
GGTTAGA19300.028.3452282
TAGAGCA20500.027.8458825
TTAGAGC21800.027.003644
GTATCAA583550.026.6562211
CATGGGT44850.026.1184484
AACGCAG607550.025.826526
CGCATAG5150.025.4206431
ACTATAG10900.024.5490932
ACATGGG553650.024.519653
TACAACG5150.024.2471942
TATGTAA24600.024.1720962
GTACATT17400.023.9397641
TATCAAC658650.023.5993672
Produced by FastQC (version 0.11.5)