FastQCFastQC Report
Fri 10 Feb 2017
ERR1148512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007836
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328960.8207920683381257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183980.4590507196402248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165100.41194300365583825No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA143970.3592212855017022No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121240.3025073880268554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70490.175880450198062No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC68530.17099003053019135No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62690.1564185760095972No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50070.12493026161749132No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48190.12023945091565623No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA44890.11200558106669037No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG42540.10614206768939646No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42520.10609216544788759No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG41950.10466995156488439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3700.044.9967042
ATGGGGG106000.038.647995
CATGGGG156500.036.738864
TGGGGGG90100.034.5123256
GGGTTAG17300.033.703111
GGTTAGA18300.029.8924262
GTTAGAG19400.029.4227393
TAGAGCA19950.027.1213975
TTAGAGC21050.026.5515164
GTAATAC6800.026.248371
GTATCAA542250.026.0807231
TACAACG5350.025.5622122
TATAACG3750.025.3695722
ACATGGG538750.025.2512173
AACGCAG569100.025.0528956
CATGGGT44600.024.5299434
GTACATG596900.024.2111781
ATCACGC7400.024.1047783
TACATGG599100.024.077752
AGTACTT236150.024.0572212-13
Produced by FastQC (version 0.11.5)