FastQCFastQC Report
Fri 10 Feb 2017
ERR1148510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4593879
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306550.6673009889899146No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA175530.3820953925865265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168880.36761960861398396No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT159020.3461562657614622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153200.33348723377346245No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG75710.16480625632499246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64180.13970764140718553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62020.1350057326281341No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59540.12960724477070468No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56920.12390400356648487No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53320.1160674889347325No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50540.1100159581913237No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47090.10250596500256103No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC45960.10004617013203874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG94500.034.4182055
CATGGGG152650.032.91494
TATCACG4300.031.8051012
TGGGGGG75850.030.415486
GGGTTAG16150.029.1087131
GTTAGAG19800.024.3249823
GGTTAGA18600.023.9764982
GTATCAA576300.023.4187051
AACGCAG593400.023.0261336
GAGTACT208650.022.66990712-13
AGTACTT220150.022.47152912-13
ACATGGG566700.022.4434833
GTAAGTA30850.021.9728872
TACATGG636800.021.569812
GTACATG635200.021.5375821
ACGCAGA644850.021.1428667
ATATGTA21750.021.0668641
TATGTAA23150.021.062032
CGCAGAG650600.021.010848
CGCATAG6250.020.9464821
Produced by FastQC (version 0.11.5)