FastQCFastQC Report
Fri 10 Feb 2017
ERR1148509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041343
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306590.7586339491599698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA201760.4992399803728612No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT184250.4559127992847922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170510.42191420030420584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142890.35357058284832543No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86310.2135676184872207No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG75980.18800680862772598No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC73250.18125162848092824No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA68140.16860731692410172No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63340.15673007710555625No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC59890.14819331098597668No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC55610.13760277214777364No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG55040.1361923499193214No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49330.12206338338517665No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48590.1202323089131509No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47750.11815379194490545No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA42760.10580641138354255No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41290.102169006689113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.035.869482
ATGGGGG90650.035.3588035
CATGGGG138600.034.538964
GGGTTAG15150.034.1735151
TGGGGGG75700.030.6350426
TACAACG6950.026.5252132
GTTAGAG19250.025.0225123
GGTTAGA19150.024.2218782
TTAGAGC21400.024.175874
TAGAGCA21850.023.6779675
GTAAGTA30050.023.153862
GAGTACT215800.023.11967812-13
AGTACTT225250.023.00773412-13
GTGGTTA25950.022.9321961
ACATGGG567050.022.610183
GTATCAA621050.022.5656261
AACGCAG630500.022.3141026
CATGGGT36450.021.8616984
GTACACT52600.021.60881
ATATGTA20950.021.5880091
Produced by FastQC (version 0.11.5)