FastQCFastQC Report
Fri 10 Feb 2017
ERR1148504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6069516
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492660.8116956936928743No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA256540.4226696164900134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256100.42194468224484455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249320.4107741045579252No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT221200.36444421598031873No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG117210.19311259744599074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107190.17660386759010108No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA97190.16012808929081002No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC96380.15879355124856742No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG95280.1569812156356454No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG76180.12551247908399946No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC75090.12371661924937673No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC73430.12098164005169441No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT68260.11246366267096092No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66600.1097286834732786No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC63860.10521432021927285No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC62920.10366559705913948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5800.042.035972
GGGTTAG25500.040.6095921
ATGGGGG139150.036.0673075
CATGGGG218900.034.7439584
GTTAGAG32150.033.292473
GGTTAGA30850.033.1541942
TGGGGGG119500.031.645476
CATGGGT99750.029.2104114
TAGAGCA37500.028.7013155
TTAGAGC37400.028.6190624
TATAACG6000.024.7773022
GTACATT27300.024.6340261
TAACGCA8450.024.6300814
GTATCAA848400.024.2293241
ACATGGG825400.023.8605463
TACAACG8750.023.786212
AACGCAG890600.023.4874256
AGTACTT333300.023.38800812-13
ATCACGC11750.022.7734663
GTACATG941850.022.6846621
Produced by FastQC (version 0.11.5)