FastQCFastQC Report
Fri 10 Feb 2017
ERR1148502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5276115
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331040.6274313581110343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178960.33918896764001544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167790.31801808717209534No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA134580.2550740459599535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT121400.23009354420819106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66860.12672202937199056No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG60530.11472456532884519No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52810.10009258706453518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4500.036.9998662
ATGGGGG109350.034.2044335
CATGGGG173150.032.728114
GGGTTAG19200.029.7557241
TGGGGGG95350.028.8723436
GTATCAA513750.027.8241371
CATGGGT60250.027.5357974
AACGCAG547800.026.4192376
ACATGGG544650.024.6631893
TCAACGC594300.024.0935764
ATCAACG590800.024.0652073
CAACGCA598500.023.9741765
TACATGG594900.023.9595532
GTACATG595450.023.9564931
TATCAAC595750.023.9453322
ACGCAGA604950.023.874257
CGCAGAG613000.023.589838
AGTACTT240600.023.58948712-13
GTTAGAG25400.022.7085023
GAGTACT227800.022.55268312-13
Produced by FastQC (version 0.11.5)