FastQCFastQC Report
Fri 10 Feb 2017
ERR1148501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7313611
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450350.6157696929738264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA252670.34547913472565056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233920.31984200417550235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225350.30812412637204795No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT224760.30731741133073665No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC115800.15833491827771534No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA113450.15512173124876344No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG105520.14427893416808743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96900.13249269068316594No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG93630.1280215751152201No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC84270.11522351954458612No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC75690.10349196860483828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG141000.036.6468775
CATGGGG224800.034.9416054
TGGGGGG117850.032.744366
TATAACG6900.031.0238482
TATCACG7300.030.1384642
GGGTTAG24850.028.2563271
CATGGGT86150.025.744824
ACATGGG796200.023.7189043
AGTACTT319250.022.55519312-13
GTAAGTA48700.022.3441772
GTATCAA844250.022.3222031
GTACATG948900.022.0615221
TACATGG945850.022.0473182
GAGTACT305750.021.96610612-13
AACGCAG883000.021.7434676
ATATGTA38050.021.7380311
TATGTAA38400.021.678992
GTACACT86750.021.3329521
CGCATAG8100.021.3045831
TACAACG9200.020.0362362
Produced by FastQC (version 0.11.5)