FastQCFastQC Report
Fri 10 Feb 2017
ERR1148500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6100635
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446230.7314484475796372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245960.4031711452988091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178220.29213352380530877No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82270.13485481429392185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76860.12598688497181032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69940.11464380347291717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA460600.039.0802151
AACGCAG516150.035.068856
TCAACGC543950.033.1896554
ATCAACG541750.033.126863
TATCAAC544700.033.090732
CAACGCA550600.032.74565
CATGGGG208600.031.7848744
ACGCAGA581050.031.121167
ATGGGGG133000.030.8054495
CGCAGAG591300.030.621918
GGTATCA304150.029.7184031
GCAGAGT623850.029.0623079
CATGGGT84400.028.8164754
GTACATG556100.028.5274371
ACATGGG538850.028.140533
TACATGG560800.028.0369782
TGGGGGG107600.025.9739786
GAGTACT266150.025.47180612-13
AGTACTT279300.024.00639512-13
TATCACG5050.023.5513822
Produced by FastQC (version 0.11.5)