FastQCFastQC Report
Fri 10 Feb 2017
ERR1148496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552153
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285730.6276810116004449No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA174410.3831373857601008No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT156280.34331007767093946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153630.33748865646651155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145380.3193653640376323No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC87930.1931613458510731No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76620.16831595950311862No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC71440.15693672862050112No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA68270.14997299080237417No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64770.14228432128709206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.13481532804367516No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG53990.11860321918002316No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50440.11080471152880847No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50350.11060700288412977No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46580.1023252074348116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG21250.040.8821831
ATGGGGG137350.037.967785
CATGGGG189000.036.8416754
GTTAGAG24150.035.4559943
TATCACG3750.034.8851432
GGTTAGA23550.034.8447382
TGGGGGG113750.034.238536
TATAACG4700.032.8945962
TTAGAGC26800.032.615714
TAGAGCA26650.032.353045
CATGGGT63900.027.451464
GTACGTT3050.027.3129351
TAACGCA6600.027.0284794
ACATGGG596200.025.7718053
GTTAAGA38350.025.428624
ATATGTA29250.025.2252791
GTACATT23650.024.9083061
TATGTAA31950.024.5670012
ACAACGC7750.024.5523223
TACATGG680200.024.3115922
Produced by FastQC (version 0.11.5)