FastQCFastQC Report
Fri 10 Feb 2017
ERR1148495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4862475
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396180.8147702558882052No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA271640.5586455457354537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT225520.4637967290320259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216110.4444444444444444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202190.41581704790255986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG122340.25160026529699386No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC109960.2261399801541396No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG108350.22282890914606243No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA101450.20863860482573174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.18648939069095471No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT83740.17221682373688296No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC79260.16300340875788566No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG77790.15998025696790213No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70800.1456048617216541No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC65520.13474619406783583No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60470.12436053655802858No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56910.11703916215507534No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC55960.11508542460372548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG113700.036.2922635
CATGGGG174650.034.929614
TGGGGGG100000.032.3448376
TATCACG5000.029.7299332
GGGTTAG15450.026.1880841
GTACATT22700.024.1148871
GTGGTTA26850.023.7117041
CTATAGC14200.023.448483
TACAACG6150.023.2038522
ATATGTA27650.023.0256521
CATGGGT47300.022.1241474
AGTACTT280650.022.04476412-13
TAACGCA6800.021.8597974
TATGTAA28650.021.7915762
ACATGGG758550.021.3598063
GAGTACT271200.021.28913112-13
GTACCGT5400.020.9202046
TATAACG4850.020.8416042
GTAAGTA41150.020.518352
GTTAAGA37900.020.3947854
Produced by FastQC (version 0.11.5)