FastQCFastQC Report
Fri 10 Feb 2017
ERR1148494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269868
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114860.9045034602021628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76420.6017948322187817No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45100.3551550239867451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.24978974192593245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31270.24624606652029973No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24860.19576837907562045No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20260.1595441415958194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.1536380159197649No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17800.14017204937836059No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT16830.1325334601706634No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15050.11851625523282734No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14080.11087766602513016No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14060.11072016934043538No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12880.10142786494344294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG954.1836756E-1156.3354642
TATAACG1101.7826096E-1048.6533552
GGGTTAG6100.040.975531
TAACGCA1703.2923708E-1034.9795384
GTTAGAG7050.034.5826033
TAGAGCA7500.032.507655
CATGGGG46250.032.0147824
ATGGGGG31050.031.7913845
TTAGAGC7750.030.6917234
GGTTAGA7300.030.1399042
CATGGGT22850.027.8458564
GCGCAGA3450.027.5998021
GTGGTTA9050.026.9612731
AACGCAG190800.026.4913166
GGTTAAG11000.026.489053
TGGGGGG25600.026.0160316
GTTAAGA12650.025.3843614
GTATCAA197100.025.091091
CTTAGCG1451.3455139E-424.6062953
ACGCAGA209050.024.0932967
Produced by FastQC (version 0.11.5)