FastQCFastQC Report
Fri 10 Feb 2017
ERR1148491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242175
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318190.9814090849506889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182450.5627395189957359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164130.5062342409030975No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA159060.4905965902519142No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126010.3886588478413411No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC79450.24505154718668798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.22688473015799582No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG68460.21115454902958664No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG61620.19005760022207313No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.1848142065126034No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC57380.1769799594408075No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52920.16322376182655163No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT47820.14749358069814245No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG46370.14302127429888886No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG45830.14135572570882202No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42320.13052965987338747No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT41400.12769205857179208No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC38690.11933347212904916No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA37630.11606406193373277No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.1124862168143299No Hit
GTACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT36290.11193103395097427No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG35830.11051223330017658No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35260.10875415423288379No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT35180.10850740629361462No Hit
GGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG34090.10514546562107227No Hit
GGTCAGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT32600.10054978525218411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG19000.042.899711
ATGGGGG122100.042.024365
CATGGGG164300.039.5900234
TGGGGGG103050.038.8870056
GGTTAGA21500.038.4410932
GTTAGAG22700.036.9316983
TATCACG3600.036.336212
TTAGAGC24100.035.279714
TAGAGCA24550.035.1174135
TACAACG3800.034.4237752
TATAACG2500.033.2971762
GTGGTTA22700.032.2378921
GTTAAGA32950.031.578294
GGTTAAG31900.029.262743
ATATGTA26050.028.7773111
CTATAGC9100.028.7486383
ACAACGC4450.028.0585563
AGAGCGC10600.027.4841828
CATGGGT32050.027.4561544
GTACATT14550.027.3967591
Produced by FastQC (version 0.11.5)