FastQCFastQC Report
Fri 10 Feb 2017
ERR1148489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4199612
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336370.8009549453616192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA199630.47535343741278957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183340.4365641397348136No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT162620.3872262485201014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155150.369438891021361No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84880.20211390957069367No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC79700.1897794367670156No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG75940.18082622870874737No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73610.1752780971194482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.16706305249151587No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT64000.15239503077903388No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC56970.13565538911689937No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG54180.12901191824387587No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48090.11451057859630841No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC46730.11127218419225396No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT42220.1005330968670439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3250.034.7629932
CATGGGG136750.033.2210164
ATGGGGG84400.032.549665
GGGTTAG14200.031.01141
TATAACG3750.030.1279262
TGGGGGG68050.029.0095756
TACAACG7100.025.1252172
GGTTAGA17700.024.5242922
GTTAGAG19050.023.7227763
TAACGCA6050.022.6057764
AGTACTT246200.022.54592112-13
GAGTACT237500.022.39538212-13
TTAGAGC19550.022.203584
AACGCAG659550.022.0876646
GTATCAA655600.022.0660171
ACAACGC8650.021.9978773
ACATGGG575700.021.700853
TAGAGCA19800.021.3225985
TTAACGC4900.020.6300264
CATGGGT43900.020.453114
Produced by FastQC (version 0.11.5)