FastQCFastQC Report
Fri 10 Feb 2017
ERR1148485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820197
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341470.7084150295102047No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA183580.38085580319642537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183270.3802126759549454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170610.35394818925450555No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT146960.3048838045415986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78120.16206806485295103No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC73990.1534999503132341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.15277383891156315No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70300.14584466153561773No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA67120.13924742080043617No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC59280.12298252540300739No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53900.11182115585732286No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG51790.10744374140724954No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50040.10381318439889489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG109600.033.092225
TATCACG5550.032.1405222
CATGGGG166350.031.6320324
TATAACG5150.031.1731832
TGGGGGG89300.030.4265926
CTATAGC13950.030.2615223
TAACGCA6100.027.2919674
GGGTTAG16450.026.4070341
TACAACG7050.026.1455152
CACTATA18050.023.0772191
GTATCAA639050.023.0370831
AGTACTT249300.022.884112-13
ACTATAG18200.022.8692152
CATGGGT46150.022.546184
AACGCAG661550.022.4231416
ACATGGG591400.022.2890193
ACAACGC7750.022.248613
GAGTACT239050.022.09936312-13
GGTTAGA18900.022.022212
CGCATAG6300.021.7245081
Produced by FastQC (version 0.11.5)