FastQCFastQC Report
Fri 10 Feb 2017
ERR1148484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030077
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187850.9253343592385905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114840.565692828400105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94320.4646129186232838No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67350.33176081498386517No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50680.24964570309402057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.20945018341668814No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30740.15142282780406852No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26650.13127580875011144No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26640.1312265495348206No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA25430.12526618448462792No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23730.11689211788518367No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22100.10886286579277535No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.10689249718114141No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21520.10600583130590613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2150.047.017972
ATGGGGG44900.036.02095
CATGGGG72400.034.083294
TATAACG2650.033.6588132
GGGTTAG9250.033.4514271
TAACGCA3350.031.9491774
TGGGGGG40300.030.688786
GGTTAGA9900.030.0322762
GTTAGAG10250.029.585463
TCACGCA3550.028.4742684
CGCATAG2353.4197E-1027.8533741
TTAGAGC11350.026.7181454
TAGAGCA11400.026.6009625
CATGGGT27100.026.5489944
AACGCAG303950.025.450516
GTTAAGA16150.025.4043774
CTATAGC4950.025.2258683
GTATCAA304700.025.2119711
TATAGCT8800.025.0006354
ACATGGG272500.024.1335163
Produced by FastQC (version 0.11.5)