FastQCFastQC Report
Fri 10 Feb 2017
ERR1148483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735097
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188231.084838484534294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.6606547069126395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72760.4193425497248857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.2974473473240977No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44870.25860225681907123No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34470.19866324476383743No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31720.18281398676846308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.1679445010855301No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29050.16742579809659056No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26270.15140363910490306No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24650.14206698530399164No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21380.12322077670585563No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA20880.12033909343396941No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.1175726774929586No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG20040.11549786553720053No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17810.10264555814458788No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17470.10068601351970524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2000.044.595112
GGGTTAG9600.040.9071431
TATAACG2050.040.6069262
TACAACG3200.037.1625942
GCGCAGA5950.036.0007861
ACAACGC3700.035.354683
ATGGGGG54750.034.752965
GGTTAGA11500.033.607912
GTTAGAG11850.033.1170433
CATGGGG82100.032.952944
TGGGGGG46800.032.2711076
GTGGTTA11500.032.078961
TTAGAGC11300.031.5717584
CATGGGT28700.031.076734
TAACGCA2950.030.2339734
GTTAAGA17750.030.1488044
TAGAGCA12100.029.484375
GGTTAAG17550.029.1371653
AACGCAG293600.027.9276356
GTATAAC4300.027.675021
Produced by FastQC (version 0.11.5)