FastQCFastQC Report
Fri 10 Feb 2017
ERR1148480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521786
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284520.8078855444368283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160910.45689885756829063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140850.3999391217978605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA122590.3480904291174989No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT102710.2916417976560756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61100.17349151822399203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51870.1472832250454741No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45530.12928099549489946No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42330.12019469666811101No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41790.11866138374109045No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA38960.11062568821614942No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38820.11022816264247744No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38310.10878003376695801No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35730.10145420533785982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG20700.035.6434481
ATGGGGG81200.035.0746465
CATGGGG125950.033.0928084
GTTAGAG23050.031.986343
GGTTAGA23300.031.3883972
TGGGGGG71450.031.1217986
TATCACG3250.031.1017992
TTAGAGC24450.029.6684464
TAGAGCA26300.028.485815
CATGGGT45450.025.7718834
ATATGTA22150.024.98261
GTATCAA482800.024.9073451
TATAACG3850.024.710372
CGCATAG3650.024.4526981
AACGCAG497900.024.3364566
ACATGGG454200.024.2180063
ACAACGC5700.023.9920183
TATGTAA22450.023.5718482
GTATAGG8000.023.0568541
GTAAGTA32150.022.932992
Produced by FastQC (version 0.11.5)