FastQCFastQC Report
Fri 10 Feb 2017
ERR1148477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024079
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310801.0277509284645012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176600.5839794529177312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156680.5181081578887324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA133550.4416220607993375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99280.3282983017308741No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68200.22552320888442398No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG60020.1984736509859696No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51470.17020058007743843No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49210.16272723034021266No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44410.1468546291284057No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42800.14153069413861213No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38920.1287003414924015No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38870.12853500189644518No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37930.1254266174924663No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37710.12469912327025849No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA37150.12284731979554768No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33610.11114127640184004No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT31280.1034364512302754No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30660.10138624024041701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2900.047.1657452
GGGTTAG18750.039.042571
GTTAGAG20100.036.0949363
GGTTAGA20800.035.4531822
ATGGGGG84850.035.042955
TATAACG2950.034.2707632
TTAGAGC21300.034.0614174
CATGGGG125150.034.0223774
CGCATAG3500.034.0092051
TGGGGGG72050.032.8442846
TACAACG3650.032.586222
TAGAGCA22500.032.5091135
CATGGGT34000.028.5096054
TCACGCA5200.026.3031024
GTACATT14450.025.5363251
AACGCAG517000.025.3933036
GTATCAA514350.025.1209261
ATCACGC5700.025.0391083
GAGTACT204600.023.36513512-13
AGTACTT212700.023.28602812-13
Produced by FastQC (version 0.11.5)