FastQCFastQC Report
Fri 10 Feb 2017
ERR1148475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084686
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266360.8634914542355364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151600.49146007081433896No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA128920.4179355694550434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128780.4174817145083811No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT101430.32881790885684964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57930.18779869328677212No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57210.18546458213250877No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51150.16581914658412558No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48740.15800635785943853No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC45950.14896167713666805No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42270.13703177568154426No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39660.1285706227473396No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38110.12354580012357821No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34290.1111620437217921No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34190.1108378616170333No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33100.1073042766751624No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA31940.10354376425996033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3900.041.1678662
ATGGGGG76350.035.4362225
CATGGGG116950.033.8625074
TAACGCA4950.033.63554
TATCACG3900.033.5441862
TGGGGGG65100.032.6973046
TACAACG4600.031.0250552
GGGTTAG10600.028.074871
GGTTAGA11600.026.6565862
GTTAGAG12750.026.5833513
GTATAGG5800.025.6546231
GTATAAC8700.024.6284371
ATATGTA19300.024.05421
TTAGAGC15100.024.0213834
AACGCAG492300.023.8169966
GTATCAA488350.023.558861
CATGGGT32600.023.3473424
ACATGGG454350.022.7197883
AGTACTT199550.022.6933412-13
GTACATT17150.022.5581171
Produced by FastQC (version 0.11.5)