FastQCFastQC Report
Fri 10 Feb 2017
ERR1148474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365281
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311210.9247667579616681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174050.5171930664928129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149200.44335079299470087No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA117220.34832158146674824No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94460.28068978489463436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.19109845507700546No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53230.15817401280903437No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC46140.13710593558160522No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44170.1312520410628414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41670.12382324091212592No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA40870.12144602486389695No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.10462722132267707No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA34560.10269573328349105No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG34420.10227972047505098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34020.10109111245093648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3150.041.531652
ATGGGGG82500.037.9133725
CATGGGG128700.036.1779374
CGCATAG3800.034.453151
TGGGGGG70500.033.2303926
GGGTTAG12850.032.881011
TATAACG3550.030.1516592
GGTTAGA15700.028.785982
GTTAGAG16550.026.9478343
GTGGTTA21950.026.8404961
GTATCAA489250.026.3340051
AACGCAG495050.026.3040986
GGTTAAG26950.026.0365733
GTTAAGA29150.025.9075164
CATGGGT41150.024.7108064
ACATGGG455150.024.6012043
TTAGAGC18400.024.2384054
ACGCAGA545950.023.8626067
CGCAGAG549850.023.6717248
TATGTAA20600.023.670842
Produced by FastQC (version 0.11.5)