FastQCFastQC Report
Fri 10 Feb 2017
ERR1148470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390426
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222530.9309219360900525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134970.5646273927743423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109090.45636217142885827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99480.416160132127077No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70400.2945081755302193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51000.21335109306876684No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45310.18954780444991814No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39310.16444767585359263No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38460.1608918243024465No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36320.1519394451030904No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.1470030864791464No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33520.14022605175813851No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30210.12637914748249895No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30040.1256679771722697No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA26610.11131907032470363No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG26380.11035689872851116No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC26370.1103150651808506No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25760.10776321877355752No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24800.10374719819814543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1950.045.740292
TATAACG2750.045.407642
GGGTTAG12350.041.4303971
TACAACG3600.041.293322
ATGGGGG68500.038.5420385
GTTAGAG14250.037.5551873
GTACATT12850.037.5033041
CATGGGG100600.036.4103624
GGTTAGA14150.035.719452
TTAGAGC15000.035.2810134
TAGAGCA15250.034.7026375
TGGGGGG56950.034.3514026
CTACGCG750.003923453431.7132669
TACATTG14850.031.2327672
TTGTAGC16200.029.3641386
ATTGTAG15500.029.1557465
GTGGTTA14100.029.1150531
ACAACGC5000.028.5419433
TAACGCA4650.028.132744
CGCATAG2552.910383E-1127.998111
Produced by FastQC (version 0.11.5)