FastQCFastQC Report
Fri 10 Feb 2017
ERR1148465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484288
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169970.6841799340495144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.3671877012649097No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86760.34923487131926734No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84910.3417880696602004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.2837835226833604No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37570.15123045315196948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.14652085426488395No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32270.12989637272329135No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30620.12325463070304249No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30240.12172501738928819No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC28530.11484175747739393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG8800.029.7504481
ATGGGGG51600.029.7312895
CATGGGG81650.029.1304724
ACGCATA2701.8189894E-1228.6301297
GTAAGTA16950.026.3108732
TGGGGGG42350.025.5541636
TATCACG2151.2407509E-724.891312
GTATCAA325350.024.5611521
TTAGAGC10950.024.4366724
AACGCAG329700.024.3296976
GGTTAGA11050.023.6774042
AACGCAT3052.7830538E-1023.395116
GAGTACT126600.023.20261612-13
GTTAGAG11000.022.7038923
TAGAGCA12500.022.3579275
TGTAAGT19450.022.3319551
TATCAAC360400.022.0097332
ACGCAGA363200.022.003777
ACATGGG296950.021.9467853
TCAACGC365050.021.908554
Produced by FastQC (version 0.11.5)