FastQCFastQC Report
Fri 10 Feb 2017
ERR1148463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682214
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286680.7785533377473444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154270.4189598974964519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126470.3434618411640388No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90620.24610193758429033No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86560.23507596245085158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63680.17293943263482242No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45250.1228880233468234No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42270.11479506622917625No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA40800.11080290281879326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA3850.041.7022137
AACGCAT4300.037.338036
TATCACG3550.035.176052
ATGGGGG78250.033.1328055
CATGGGG121650.033.043854
TGGGGGG64050.029.2447196
GTATCAA446350.028.812271
AACGCAG454450.028.171766
GGGTTAG13450.027.8751031
CGCATAG5850.026.4285648
TATCAAC501450.025.6382
GGTTAGA14450.025.514082
ATCAACG504850.025.3239923
ACGCAGA505800.025.2529147
TCAACGC509400.025.214534
CGCAGAG509200.025.0609448
CAACGCA514400.025.0272455
GCAGAGT534000.023.8191159
GTACACT44650.023.7244851
ACATGGG419100.023.5530153
Produced by FastQC (version 0.11.5)