FastQCFastQC Report
Fri 10 Feb 2017
ERR1148461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496248
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220890.6317915662733307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121830.3484592626152378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88620.253471721685647No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79670.2278728511249774No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79370.22701478842461978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48200.1378620738574609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35480.10148021536229695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2950.042.3327037
TATCACG3400.029.7336832
GTATCAA356150.028.0328711
CATGGGG106000.027.6579344
AACGCAG363400.027.3772436
ATGGGGG67550.027.2907225
AACGCAT4700.025.3052646
TATCAAC398000.024.9972112
TCAACGC403800.024.8737954
ATCAACG398850.024.8544793
ACGCAGA402650.024.6937647
CAACGCA407050.024.6751945
CGCAGAG407050.024.4268348
GCAGAGT426650.023.3046869
GGGTTAG10400.022.8841721
GAGTACT157100.022.84472812-13
TGGGGGG53850.022.748896
ACATGGG339000.022.7344263
GTTAAGA26500.022.6649194
GTACATG373500.022.0153711
Produced by FastQC (version 0.11.5)