FastQCFastQC Report
Fri 10 Feb 2017
ERR1148459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915641
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200150.686469973498109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108960.37370856014166354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81600.2798698468021269No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78950.27078093633612643No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76970.26398997681813363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.14391346534089758No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36910.12659308879248166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29820.10227596607401254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA3200.037.164857
AACGCAT3700.032.1425746
ATGGGGG60350.031.924755
TATCACG2800.031.856132
CATGGGG96000.031.1570634
GTATCAA324300.029.4337121
CGCATAG3850.029.3457498
GGGTTAG12100.029.0171181
AACGCAG334450.028.1983916
TGGGGGG50450.027.3450786
TATCAAC367150.025.7845342
ATCAACG365550.025.7835223
TCAACGC369350.025.7275524
GGTTAGA12800.025.551272
CAACGCA372400.025.516845
ATATGTA16200.025.3467161
ACGCAGA373050.025.2487837
GTAAGTA23200.025.1187422
CGCAGAG376950.025.0506538
TATGTAA17200.024.5465072
Produced by FastQC (version 0.11.5)