FastQCFastQC Report
Fri 10 Feb 2017
ERR1148458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794430
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215240.7702465261251847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121470.4346861435069048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93920.3360971647169548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71540.25600927559466513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66970.23965531432170425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.17030306717291183No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40800.146004730839563No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34330.12285152965005386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2450.038.834447
ATGGGGG75850.033.7113385
CATGGGG112550.033.444244
TGGGGGG60050.031.1932546
GGGTTAG11300.030.5416551
TATCACG2750.030.2732122
TATAACG2201.6007107E-1029.7326182
GTATCAA336950.029.4736441
AACGCAG350200.028.3232486
AACGCAT3500.027.1841096
TAACGCA3600.026.4289934
TATCAAC380550.026.1425132
ATCAACG383400.025.8241043
TCAACGC387150.025.6200494
GTTAGAG12800.025.5514683
GGTTAGA12800.025.5514682
CAACGCA391100.025.4373175
ACGCAGA390900.025.3590567
CGCAGAG395500.025.049078
TACAACG4100.024.6563152
Produced by FastQC (version 0.11.5)