FastQCFastQC Report
Fri 10 Feb 2017
ERR1148457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735380
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271100.7257628407283864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144330.3863863917459535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117040.3133282289887508No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77620.20779679711301124No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75540.2022284212048038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59260.15864517130787228No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38200.10226536523727171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA3350.031.951017
TATCACG3200.031.590452
ATGGGGG81600.031.4815655
CATGGGG125050.031.2419824
GTATCAA406500.030.4791431
AACGCAG420850.029.5591036
AACGCAT4300.027.657856
TGGGGGG65700.027.3337046
TATCAAC460600.026.8532142
TCAACGC464650.026.7343334
ATCAACG460450.026.6940773
ACGCAGA467800.026.5288937
CAACGCA471300.026.3700875
CGCAGAG471700.026.3473728
TACAACG5900.026.2046412
GTGGTTA23200.025.1374171
GCAGAGT498200.024.9339799
ACATGGG390550.024.3917373
ATATGTA20850.024.260261
GTTAAGA33600.024.0689144
Produced by FastQC (version 0.11.5)