FastQCFastQC Report
Fri 10 Feb 2017
ERR1148455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856792
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234190.8197656672239351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130610.45719114307236924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101730.35609872892391187No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72180.25266102677408786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69690.24394495644065092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.1868879498402404No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36680.12839576700018762No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA32920.11523415075371254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG53800.032.273535
CATGGGG90800.031.041194
TGGGGGG44750.030.030416
AACGCAG367650.029.1451346
ACGCATA2052.4447218E-929.0062777
GTATCAA372100.028.9487481
CATGGGT34150.026.6407594
TATCACG3150.026.427942
ACGCAGA412400.025.9537267
TATCAAC415450.025.9206282
CGCAGAG414850.025.728788
ATCAACG416400.025.704413
TCAACGC420000.025.5407164
CAACGCA425800.025.2626425
GCAGAGT432050.024.7320389
ACATGGG344800.023.7644523
GTAAGTA17750.023.4501462
GTACATG374650.023.160221
TACATGG375650.022.9683532
GAGTACT164350.022.92123212-13
Produced by FastQC (version 0.11.5)