FastQCFastQC Report
Fri 10 Feb 2017
ERR1148454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942262
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160230.824965941773046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95310.49071649447911764No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86570.4457174160849566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79580.40972845064157154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.3563885819729779No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40680.20944651133575182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.1973987031615714No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35200.18123198620989342No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30540.15723934258097003No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28500.14673612519835122No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27070.1393735757585743No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26280.13530615334079543No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25560.13159913544104762No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24880.12809806298017468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22940.11810970919474305No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA21810.11229175054652771No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21650.11146796879102819No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC19580.10081029232925322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2200.043.2456637
TATCACG2300.041.3654172
AACGCAT2700.035.237216
ATGGGGG37450.033.9787375
CATGGGG64500.031.5291064
GTTAGAG7350.028.3156133
CGCATAG3250.027.4443638
TGGGGGG33600.027.2537786
GGGTTAG7050.027.003411
CATGGGT26750.026.2303124
GGTTAGA7350.025.8885612
TATAACG2308.340066E-925.8533862
TAACGCA2601.1150405E-925.1573334
TACAACG2651.3897079E-924.6826672
ATAAGTA5900.024.1882515
TTAGAGC9150.023.3951954
ATTAACG2352.9283547E-722.7729843
TAGAGCA9250.022.4994345
TCACGCA4250.022.385994
TATGTAA9350.022.2587972
Produced by FastQC (version 0.11.5)