FastQCFastQC Report
Fri 10 Feb 2017
ERR1148453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715537
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156220.9106186575981747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91740.5347596700042028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73070.42593077269682905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67750.39492007458888967No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67580.39392913122829765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.2067574176482349No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34770.20267706263403235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29180.17009251330632916No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24310.14170490056466284No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23980.13978130462939592No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22870.13331102739258902No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22000.12823972901779443No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA21620.12602467915294163No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21260.12392621085992317No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20550.11978756505980344No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20470.119321238772466No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20300.11833029541187395No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18180.10597264879743193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1850.054.645842
ACGCATA2350.037.9579967
AACGCAT2450.036.408696
ATGGGGG37450.030.646825
CATGGGG59800.030.2309884
TCACGCA3400.029.7337684
TAACGCA2404.4019544E-1027.2559534
TGGGGGG31550.026.3881266
CGCATAG3400.026.2356788
GTATCAA301800.024.6095961
AACGCAG297350.024.5589796
ATCACGC3650.024.4387113
CATGGGT22650.023.8920334
GTACCGT1250.001639053223.7870146
GTACACT19950.022.6714781
GAGTACT119400.022.66141312-13
GTATCAC5000.022.61481
AGTACTT124350.022.40493612-13
GTACATT8300.022.2276081
TATCAAC333050.022.1943072
Produced by FastQC (version 0.11.5)