FastQCFastQC Report
Fri 10 Feb 2017
ERR1148451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7936241
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553970.6980256773956336No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT299890.37787410941779614No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA267290.3367967278211435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227940.28721406015769935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220510.2778519452723273No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG146480.1845710078612784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113760.14334242117899393No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC109130.13750842495836504No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA107990.13607197664486245No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG102550.1292173460962186No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC100020.1260294388741471No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC99870.12584043251710728No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC82980.10455831671442437No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA79940.1007277878784175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA7400.039.3748137
TATCACG7250.031.9875352
ATGGGGG183950.031.8736135
AACGCAT9450.031.4624396
CATGGGG277800.031.0591324
TGGGGGG146100.028.8162336
GTATCAA1068700.026.1912251
TACAACG11300.025.7852782
AACGCAG1082450.025.5501026
CATGGGT100000.025.034354
TATCAAC1175050.023.6783182
ATCAACG1194300.023.0925273
ACGCAGA1194850.023.0769217
TCAACGC1206700.023.0424884
CAACGCA1214250.022.92375
CGCAGAG1207950.022.8365028
CGCATAG12550.022.7432068
AGTACTT411600.022.55182512-13
GTAAGTA67550.022.5355822
GGGTTAG25450.022.4454211
Produced by FastQC (version 0.11.5)