FastQCFastQC Report
Fri 10 Feb 2017
ERR1148450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867980
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134720.7212068651698625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97600.522489534149188No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94530.5060546686795362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80790.4324992772941894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.3140825918907054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45780.2450775704236662No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37100.19861026349318514No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36960.19786079080075805No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36710.19652244670713817No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31670.16954142977976208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.16638293771881923No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30100.16113662887182947No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT29430.15754986670092827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28060.1502157410678915No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27070.14491589845715694No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24560.13147892375721368No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24500.13115772117474492No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA20410.10926241180312422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2700.048.4517862
TAACGCA2500.040.435224
TATAACG1900.037.5559272
ACGCATA1601.70985E-1037.163737
GTATAAC3550.036.8782921
GGGTTAG5850.032.551471
TCACGCA4450.032.0702324
ATGGGGG44250.031.7138985
CATGGGG66650.031.4938244
TGGGGGG36650.030.177156
AACGCAT2252.0736479E-1029.0702926
GGTTAGA6500.028.3595432
CTATAGC3700.027.3210933
GTATCAC6100.027.3152221
ATCACGC4950.026.4282463
GTTAGAG7200.024.7764823
ATATGTA9150.024.713771
GTACATT8800.022.9917891
TATGTAA10550.022.5454242
CGCATAG2653.754758E-822.4384788
Produced by FastQC (version 0.11.5)