FastQCFastQC Report
Fri 10 Feb 2017
ERR1148448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440643
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183180.7505399191934257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105850.43369718553676223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.33962361557999265No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51560.21125580431058535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47290.19376041477594225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.17835463851124478No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32840.13455470546081505No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26660.10923350936617932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2200.037.8375632
GGGTTAG7900.036.9076271
ACGCATA2450.033.976597
ATGGGGG59600.033.021715
CATGGGG89000.033.0030984
GTTAGAG9750.030.4918083
GTATCAA272450.029.1132351
TGGGGGG49250.028.9749576
GTGGTTA14700.028.33531
AACGCAG281050.028.2009896
TATAACG1903.7465725E-828.1648032
GTTAAGA20850.027.9471684
TTAACGC3000.027.7475454
GGTTAGA10150.027.5327512
CGCATAG2905.456968E-1226.6540458
GGTTAAG20550.026.3297863
ATATGTA13750.025.965441
TATGTAA14000.025.9071482
AGCGTAA1150.001009185625.8517516
ATCAACG306550.025.564183
Produced by FastQC (version 0.11.5)