FastQCFastQC Report
Fri 10 Feb 2017
ERR1148447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302051
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183510.7971587076046535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104500.45394302732650144No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92520.4019024773995016No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89280.3878280715761727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79290.3444319869542421No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45040.19565161675392942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44120.1916551805324904No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37270.16189910649242784No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36360.15794610979513485No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35470.15407999214613405No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30070.1306226491072526No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC29170.12671309193410574No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28880.1254533457338695No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28470.12367232524388035No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25450.1105535889517652No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23950.10403766032985368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2250.039.642697
TATCACG2500.038.0578082
GGGTTAG8300.037.9982451
ATGGGGG45150.031.3460645
CATGGGG76950.030.2156494
AACGCAT3150.030.2039536
GTTAGAG10250.030.1677743
TTAGAGC10400.029.7326624
TAGAGCA11850.028.6035755
TATAACG2751.8189894E-1228.110882
CATGGGT34000.027.4589884
GGTTAGA11200.027.077962
GTTAAGA18950.026.0454964
GTAAGTA16450.025.6658842
TGGGGGG38450.025.5177256
AGTACTC12800.025.0869335
CGCATAG3351.8189894E-1224.8506418
GGTTAAG19350.024.2778323
ATTAACG2451.6327249E-824.271563
TGTAAGT17300.024.077851
Produced by FastQC (version 0.11.5)