FastQCFastQC Report
Fri 10 Feb 2017
ERR1148446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819068
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128180.7046465552689619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76620.4212047048268674No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59720.3282999865865377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.30872952522940317No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51010.28041832410882933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.18135660678985063No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27410.15068155780872403No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26840.14754808506334013No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23920.13149590889400506No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21530.11835731264581643No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20770.11417934898530456No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20480.11258512600958294No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC18840.1035695202158468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2150.047.0158652
ATGGGGG48150.032.972285
CATGGGG69050.031.8619274
TACAACG2450.031.5508652
TATAACG1551.9831896E-730.6896742
TGGGGGG37400.030.0485956
TAACGCA2502.3646862E-1128.5413974
GGGTTAG8850.028.2429621
TCACGCA3600.028.078924
CGCATAG2603.6379788E-1127.4670541
ATCACGC3900.027.4436513
GGTTAGA8750.027.1822832
CATGGGT21100.026.4898434
GTTAGAG9100.026.136813
ACGCATA1608.78934E-626.0142947
AACGCAT1608.78934E-626.0142946
ACAACGC3250.025.6140733
GCGGTTA4750.025.0576631
TAGAGCA9500.025.0363125
ATATGTA11000.024.8868161
Produced by FastQC (version 0.11.5)