FastQCFastQC Report
Fri 10 Feb 2017
ERR1148445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734260
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214370.7840146877034372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120880.442094021782859No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114000.4169318206754295No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106890.39092844133330407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95720.35007643750045714No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53100.19420245331460798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50360.1841814604317073No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41980.15353331431539063No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41420.1514852281787394No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40460.14797422337305158No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35550.13001689671062738No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35540.12998032374390145No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32200.1177649528574459No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31910.11670433682239435No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31860.1165214719887648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2550.039.6429062
ACGCATA3050.033.143467
ATGGGGG51000.030.6643925
CATGGGG84650.029.1521054
AACGCAT3500.028.8821586
TATAACG2950.028.2203752
GGGTTAG9400.027.8516981
TAACGCA4150.027.224154
CATGGGT34750.026.865424
TGGGGGG41350.026.6038826
GCGATTA3900.025.9364871
CGCATAG3900.025.9198888
GTAAGTA17300.025.4356232
CTATAGC7900.024.8390873
CGATTAA4300.023.5091652
AACGCAG432850.023.2028356
TGTAAGT19300.023.1222881
GTATCAA438800.022.929991
GAGTACT164000.022.80634112-13
AGTACTT168800.021.98168412-13
Produced by FastQC (version 0.11.5)