FastQCFastQC Report
Fri 10 Feb 2017
ERR1148444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189870
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153530.7010918456346724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90890.41504746857119373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA78420.3581034490631864No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT73270.33458607131930207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67770.3094704251850567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38560.17608351180663692No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36060.16466730901834356No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33440.1527031284962121No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31870.14553375314516387No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31780.1451227698447853No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30210.13795339449373709No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26450.12078342550014384No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC26020.1188198386205574No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24680.11270075392603215No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23210.10598802668651564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1800.046.2512557
ATGGGGG49350.036.510985
TATCACG2450.036.4076962
CATGGGG73650.035.0416834
AACGCAT2500.033.3009036
GTTAGAG10950.031.497923
CGCATAG2650.031.4159478
TGGGGGG42300.031.2090556
GGGTTAG10550.031.0252671
GGTTAGA11150.029.866282
TATAACG1704.4499757E-727.9839532
TAGAGCA12150.026.9187185
TACAACG3600.026.4292872
CATGGGT30300.026.298454
AGTACTC13150.026.2283065
TTAGAGC13100.025.4205364
TATGTAA13550.025.0151752
TAACGCA3351.8189894E-1224.8514214
ATCACGC3600.024.7774563
TCACGCA4350.024.6065794
Produced by FastQC (version 0.11.5)